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human tlr8 reporter hek293 cells  (InvivoGen)


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    InvivoGen human tlr8 reporter hek293 cells
    Human Tlr8 Reporter Hek293 Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+tlr8+reporter+hek293+cells/HEK-Blue+hTLR8+cells/pm40592817-280-9-18
    Average 95 stars, based on 90 article reviews
    human tlr8 reporter hek293 cells - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Orchestrating intratumoral DC-T cell immunity for enhanced tumor control via radiotherapy-activated TLR7/8 prodrugs in mice.
    Article Snippet: Liang's Lab (Institute of Biophysics, Chinese Academy of Sciences) and cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillinstreptomycin. .. RAW-Lucia ISG cells (#rawl-isg), RAW-Lucia ISG-KOSTING cells (#rawl-kostg) and Human TLR8 reporter HEK293 cells (#hkb-htlr8) were purchased from InvivoGen. .. RAW-Blue reporter cells were kindly provided by Dr. Zhibo Liu's Lab. All cells, 4T1-luc2 excepted, were cultured in DMEM supplemented with 10% FBS and

    Activation Assay:

    Article Title: Orchestrating intratumoral DC-T cell immunity for enhanced tumor control via radiotherapy-activated TLR7/8 prodrugs in mice.
    Article Snippet: Liang's Lab (Institute of Biophysics, Chinese Academy of Sciences) and cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillinstreptomycin. .. RAW-Lucia ISG cells (#rawl-isg), RAW-Lucia ISG-KOSTING cells (#rawl-kostg) and Human TLR8 reporter HEK293 cells (#hkb-htlr8) were purchased from InvivoGen. .. RAW-Blue reporter cells were kindly provided by Dr. Zhibo Liu's Lab. All cells, 4T1-luc2 excepted, were cultured in DMEM supplemented with 10% FBS and

    Incubation:

    Article Title: Orchestrating intratumoral DC-T cell immunity for enhanced tumor control via radiotherapy-activated TLR7/8 prodrugs in mice.
    Article Snippet: Liang's Lab (Institute of Biophysics, Chinese Academy of Sciences) and cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillinstreptomycin. .. RAW-Lucia ISG cells (#rawl-isg), RAW-Lucia ISG-KOSTING cells (#rawl-kostg) and Human TLR8 reporter HEK293 cells (#hkb-htlr8) were purchased from InvivoGen. .. RAW-Blue reporter cells were kindly provided by Dr. Zhibo Liu's Lab. All cells, 4T1-luc2 excepted, were cultured in DMEM supplemented with 10% FBS and

    Irradiation:

    Article Title: Orchestrating intratumoral DC-T cell immunity for enhanced tumor control via radiotherapy-activated TLR7/8 prodrugs in mice.
    Article Snippet: Liang's Lab (Institute of Biophysics, Chinese Academy of Sciences) and cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillinstreptomycin. .. RAW-Lucia ISG cells (#rawl-isg), RAW-Lucia ISG-KOSTING cells (#rawl-kostg) and Human TLR8 reporter HEK293 cells (#hkb-htlr8) were purchased from InvivoGen. .. RAW-Blue reporter cells were kindly provided by Dr. Zhibo Liu's Lab. All cells, 4T1-luc2 excepted, were cultured in DMEM supplemented with 10% FBS and

    Marker:

    Article Title: Orchestrating intratumoral DC-T cell immunity for enhanced tumor control via radiotherapy-activated TLR7/8 prodrugs in mice.
    Article Snippet: Liang's Lab (Institute of Biophysics, Chinese Academy of Sciences) and cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillinstreptomycin. .. RAW-Lucia ISG cells (#rawl-isg), RAW-Lucia ISG-KOSTING cells (#rawl-kostg) and Human TLR8 reporter HEK293 cells (#hkb-htlr8) were purchased from InvivoGen. .. RAW-Blue reporter cells were kindly provided by Dr. Zhibo Liu's Lab. All cells, 4T1-luc2 excepted, were cultured in DMEM supplemented with 10% FBS and

    Flow Cytometry:

    Article Title: Orchestrating intratumoral DC-T cell immunity for enhanced tumor control via radiotherapy-activated TLR7/8 prodrugs in mice.
    Article Snippet: Liang's Lab (Institute of Biophysics, Chinese Academy of Sciences) and cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillinstreptomycin. .. RAW-Lucia ISG cells (#rawl-isg), RAW-Lucia ISG-KOSTING cells (#rawl-kostg) and Human TLR8 reporter HEK293 cells (#hkb-htlr8) were purchased from InvivoGen. .. RAW-Blue reporter cells were kindly provided by Dr. Zhibo Liu's Lab. All cells, 4T1-luc2 excepted, were cultured in DMEM supplemented with 10% FBS and

    Immunopeptidomics:

    Article Title: Orchestrating intratumoral DC-T cell immunity for enhanced tumor control via radiotherapy-activated TLR7/8 prodrugs in mice.
    Article Snippet: Liang's Lab (Institute of Biophysics, Chinese Academy of Sciences) and cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillinstreptomycin. .. RAW-Lucia ISG cells (#rawl-isg), RAW-Lucia ISG-KOSTING cells (#rawl-kostg) and Human TLR8 reporter HEK293 cells (#hkb-htlr8) were purchased from InvivoGen. .. RAW-Blue reporter cells were kindly provided by Dr. Zhibo Liu's Lab. All cells, 4T1-luc2 excepted, were cultured in DMEM supplemented with 10% FBS and

    Injection:

    Article Title: Orchestrating intratumoral DC-T cell immunity for enhanced tumor control via radiotherapy-activated TLR7/8 prodrugs in mice.
    Article Snippet: Liang's Lab (Institute of Biophysics, Chinese Academy of Sciences) and cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillinstreptomycin. .. RAW-Lucia ISG cells (#rawl-isg), RAW-Lucia ISG-KOSTING cells (#rawl-kostg) and Human TLR8 reporter HEK293 cells (#hkb-htlr8) were purchased from InvivoGen. .. RAW-Blue reporter cells were kindly provided by Dr. Zhibo Liu's Lab. All cells, 4T1-luc2 excepted, were cultured in DMEM supplemented with 10% FBS and

    Two Tailed Test:

    Article Title: Orchestrating intratumoral DC-T cell immunity for enhanced tumor control via radiotherapy-activated TLR7/8 prodrugs in mice.
    Article Snippet: Liang's Lab (Institute of Biophysics, Chinese Academy of Sciences) and cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillinstreptomycin. .. RAW-Lucia ISG cells (#rawl-isg), RAW-Lucia ISG-KOSTING cells (#rawl-kostg) and Human TLR8 reporter HEK293 cells (#hkb-htlr8) were purchased from InvivoGen. .. RAW-Blue reporter cells were kindly provided by Dr. Zhibo Liu's Lab. All cells, 4T1-luc2 excepted, were cultured in DMEM supplemented with 10% FBS and



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    MEDI9197 activates innate and adaptive immune cells. a SEAP reporter activity in HEK293-NFκB-SEAP cells expressing human TLR7 or <t>TLR8.</t> Results are shown as fold-change relative to DMSO. b pDC and mDC enriched from peripheral blood and treated with MEDI9197 or DMSO. IFN-α and IL-12p40 secretion was measured from pDC and mDC, respectively, by ELISA. Results are shown as mean ± SEM, n = 3 donors. c IL-12p70 cytokine release from 20 ng/mL LPS or 3 μM MEDI9197-treated monocyte derived macrophages was tested by ELISA. Results show the mean of 5 donors. d Median fluorescence intensity (MFI) of activation markers on cell subsets from human PBMCs stimulated with MEDI9197 or DMSO. NK cells = CD3 − CD19 − CD56 high ; B cells = CD3 − CD19 + ; pDC = CD3 − CD19 − CD20 − CD56 − HLA-DR + CD14 − CD16 − CD123 + BDCA4 + ; monocytes = CD3 − CD19 − CD20 − CD56 − HLA − DR + ; Data show the mean ± SEM, representative of 4 donors. e IL-5 release (ELISA) from human PBMCs stimulated with MEDI9197 or DMSO with or without PHA-L. Data show mean of technical triplicates ±SEM, representative of 9 donors. f Cytokine production (ELISA and MSD) from human PBMCs stimulated with MEDI9197, C class CpG, or STING agonist. Results show mean ± SEM of 3 donors, representative of 6 donors. g Percent specific killing of Eu-loaded K562 target cells co-cultured with NK cells isolated from human PBMCs stimulated with MEDI9197 or DMSO. Data show mean of technical duplicates ±SEM, 9 donors. h and i Proportion of CMV peptide-specific CD8 cells ( h ) or granzyme B-positive CD8 cells ( i ) after stimulation of PBMCs with MEDI9197 or DMSO and a titration of CMV peptide, 2 donors. Data are representative of ≥2 independent experiments. Statistical analyses were performed by one-way (C) and two-way (F-I) ANOVA with Tukey’s (C, F, H, I) or Sidak’s (G) post hoc test or Welch’s upaired T test (one-tailed) (B, D). * p < 0.05, ** p < 0.01, *** P < 0.001, **** p < 0.0001
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    Image Search Results


    MEDI9197 activates innate and adaptive immune cells. a SEAP reporter activity in HEK293-NFκB-SEAP cells expressing human TLR7 or TLR8. Results are shown as fold-change relative to DMSO. b pDC and mDC enriched from peripheral blood and treated with MEDI9197 or DMSO. IFN-α and IL-12p40 secretion was measured from pDC and mDC, respectively, by ELISA. Results are shown as mean ± SEM, n = 3 donors. c IL-12p70 cytokine release from 20 ng/mL LPS or 3 μM MEDI9197-treated monocyte derived macrophages was tested by ELISA. Results show the mean of 5 donors. d Median fluorescence intensity (MFI) of activation markers on cell subsets from human PBMCs stimulated with MEDI9197 or DMSO. NK cells = CD3 − CD19 − CD56 high ; B cells = CD3 − CD19 + ; pDC = CD3 − CD19 − CD20 − CD56 − HLA-DR + CD14 − CD16 − CD123 + BDCA4 + ; monocytes = CD3 − CD19 − CD20 − CD56 − HLA − DR + ; Data show the mean ± SEM, representative of 4 donors. e IL-5 release (ELISA) from human PBMCs stimulated with MEDI9197 or DMSO with or without PHA-L. Data show mean of technical triplicates ±SEM, representative of 9 donors. f Cytokine production (ELISA and MSD) from human PBMCs stimulated with MEDI9197, C class CpG, or STING agonist. Results show mean ± SEM of 3 donors, representative of 6 donors. g Percent specific killing of Eu-loaded K562 target cells co-cultured with NK cells isolated from human PBMCs stimulated with MEDI9197 or DMSO. Data show mean of technical duplicates ±SEM, 9 donors. h and i Proportion of CMV peptide-specific CD8 cells ( h ) or granzyme B-positive CD8 cells ( i ) after stimulation of PBMCs with MEDI9197 or DMSO and a titration of CMV peptide, 2 donors. Data are representative of ≥2 independent experiments. Statistical analyses were performed by one-way (C) and two-way (F-I) ANOVA with Tukey’s (C, F, H, I) or Sidak’s (G) post hoc test or Welch’s upaired T test (one-tailed) (B, D). * p < 0.05, ** p < 0.01, *** P < 0.001, **** p < 0.0001

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Intratumoral immunotherapy with TLR7/8 agonist MEDI9197 modulates the tumor microenvironment leading to enhanced activity when combined with other immunotherapies

    doi: 10.1186/s40425-019-0724-8

    Figure Lengend Snippet: MEDI9197 activates innate and adaptive immune cells. a SEAP reporter activity in HEK293-NFκB-SEAP cells expressing human TLR7 or TLR8. Results are shown as fold-change relative to DMSO. b pDC and mDC enriched from peripheral blood and treated with MEDI9197 or DMSO. IFN-α and IL-12p40 secretion was measured from pDC and mDC, respectively, by ELISA. Results are shown as mean ± SEM, n = 3 donors. c IL-12p70 cytokine release from 20 ng/mL LPS or 3 μM MEDI9197-treated monocyte derived macrophages was tested by ELISA. Results show the mean of 5 donors. d Median fluorescence intensity (MFI) of activation markers on cell subsets from human PBMCs stimulated with MEDI9197 or DMSO. NK cells = CD3 − CD19 − CD56 high ; B cells = CD3 − CD19 + ; pDC = CD3 − CD19 − CD20 − CD56 − HLA-DR + CD14 − CD16 − CD123 + BDCA4 + ; monocytes = CD3 − CD19 − CD20 − CD56 − HLA − DR + ; Data show the mean ± SEM, representative of 4 donors. e IL-5 release (ELISA) from human PBMCs stimulated with MEDI9197 or DMSO with or without PHA-L. Data show mean of technical triplicates ±SEM, representative of 9 donors. f Cytokine production (ELISA and MSD) from human PBMCs stimulated with MEDI9197, C class CpG, or STING agonist. Results show mean ± SEM of 3 donors, representative of 6 donors. g Percent specific killing of Eu-loaded K562 target cells co-cultured with NK cells isolated from human PBMCs stimulated with MEDI9197 or DMSO. Data show mean of technical duplicates ±SEM, 9 donors. h and i Proportion of CMV peptide-specific CD8 cells ( h ) or granzyme B-positive CD8 cells ( i ) after stimulation of PBMCs with MEDI9197 or DMSO and a titration of CMV peptide, 2 donors. Data are representative of ≥2 independent experiments. Statistical analyses were performed by one-way (C) and two-way (F-I) ANOVA with Tukey’s (C, F, H, I) or Sidak’s (G) post hoc test or Welch’s upaired T test (one-tailed) (B, D). * p < 0.05, ** p < 0.01, *** P < 0.001, **** p < 0.0001

    Article Snippet: Human TLR7, human TLR8, or mouse TLR7 HEK293 reporter cells (InvivoGen) were seeded at 3 × 10 4 cells/96-well for 24 h then treated with MEDI9197 or DMSO for 24 h. Secreted alkaline phosphatase (SEAP) was measured from supernatants using QuantiBlue reagent (InvivoGen).

    Techniques: Activity Assay, Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay, Fluorescence, Activation Assay, Cell Culture, Isolation, Titration, One-tailed Test